This document highlights best practices, examples, and resources for designing effective wildlife crossing projects in accordance with the criteria for the federal “Wildlife Crossings Pilot Program.” The Infrastructure Investment and Jobs Act (H.R. 3684), which became law in November 2021 (Public Law 117-58, also known as the “Bipartisan Infrastructure Law”), provided new federal funding for projects and research to reduce wildlife-vehicle collisions (WVCs) and improve habitat connectivity. One of the key provisions is the establishment of a new, $350-million (total) Wildlife Crossings Pilot Program (WCPP) for fiscal years 2022 through 2026.